广州中山大学2010-2发现:水稻转基因后生化成分有重大改变
原文英文标题: Unintended compositional changes in transgenic rice seeds ( Oryza sativa L.) studied by spectral and chromatographic analysis coupled with chemometrics methods. 作者工作单位: School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, Guangdong 510275, China. 中山大学化学与化学工程学院 作者:Zhe Jiao; Xiao-xi Si; Gong-ke Li; Zhuo-min Zhang; Xin-ping Xu 期刊名称和期号: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO 发表日期:2010年2月 http://www.biomedsearch.com/nih/Unintended-Compositional-Changes-in-Transgenic/20050687.html 摘要英文原文: Unintended compositional changes in transgenic rice seeds were studied by near-infrared reflectance, GC-MS, HPLC, and ICP-AES coupled with chemometrics strategies. Three kinds of transgenic rice with resistance to fungal diseases or insect pests were comparatively studied with the nontransgenic counterparts in terms of key nutrients such as protein, amino acids, fatty acids, vitamins, elements, and antinutrient phytic acid recommended by the Organization for Economic Co-operation and Development (OECD). The compositional profiles were discriminated by chemometrics methods, and the discriminatory compounds were protein, three amino acids, two fatty acids, two vitamins, and several elements. Significance of differences for these compounds was proved by analysis of variance, and the variation extent ranged from 20 to 74% for amino acids, from 19 to 38% for fatty acids, from 25 to 57% for vitamins, from 20 to 50% for elements, and 25% for protein, whereas phytic acid content did not change significantly. The unintended compositional alterations as well as unintended change of physical characteristic in transgenic rice compared with nontransgenic rice might be related to the genetic transformation, the effect of which needs to be elucidated by additional studies. |
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